Tris (Hydroxymethyl) Aminomethane
CAS 77-86-1·C₄H₁₁NO₃
Tris base in AR and molecular-biology grades — the standard buffer for pH 7 to 9 biological work.
Grades offered
- ARAnalytical ReagentHigh purity with certified assay and stated trace-impurity limits.
- MOLECULAR BIOLOGYFor Molecular BiologyCertified free of DNase, RNase and protease.
- FOR BIOCHEMISTRYFor BiochemistryControlled for the contaminants that inhibit biological systems.
Typical applications
- TAE and TBE electrophoresis buffers
- Protein purification buffers
- Enzyme assay buffers
Tris (Hydroxymethyl) Aminomethane
C₄H₁₁NO₃ · M.W. 121.14 g/mol
- CAS
- 77-86-1
- Assay
- ≥ 99.8%
- Batch
- Use before
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Pack sizes
- 100 g
- 500 g
- 1 kg
Worth knowing before you order
Tris pKa shifts by roughly −0.03 per °C. A buffer titrated to pH 8.0 at room temperature sits nearer pH 8.6 at 4 °C — adjust at the working temperature, not the bench temperature.Identification
| CAS number | 77-86-1 |
|---|---|
| Molecular formula | C₄H₁₁NO₃ |
| Molecular weight | 121.14 g/mol |
| Assay | ≥ 99.8% |
| Also known as | Tris base, THAM, Trometamol, Trizma base |
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