Gradient PCR Thermal Cycler, 96-Well
Gradient thermal cycler for amplifying DNA and RNA, running a row of different annealing temperatures across the block so a primer pair can be optimised in one run.
Typical applications
- End-point PCR
- Annealing temperature optimisation
- Gene expression analysis
- Genotyping
- Pathogen detection
- Template preparation for DNA sequencing
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Worth knowing before you order
A thermal cycler is bought on three things — block format, ramp rate and gradient range — and only one of them survives this scan cleanly. The maximum ramping rate is printed, 6 °C/s, and that is the figure that sets run time. The block format is 96-well, taken from the model designation and from the 96-position plate layout on the instrument's own screen; there is no legible specification row confirming it, and the tube format the block accepts is not stated at all. The gradient span is nowhere legible, and it is the figure a gradient cycler exists for: a wide span brackets an unknown annealing temperature across a single plate, a narrow one only confirms a guess you have already made. Ask in writing for the gradient range, the block temperature range, the hot-lid range, the block uniformity and the tube format before ordering. Finally, the catalogue reproduces screen images carrying entered run values such as lid temperature and reaction volume — those are somebody's example file, not the instrument's limits, and none of them have been copied into the rows above.Specification
| Block format | 96-well, from the model designation and the 96-position plate layout shown on the instrument screen |
|---|---|
| Maximum ramping rate | 6 °C/s |
| Run modes | Standard and gradient, selected per programme file |
| Hot lid | Temperature and work mode both settable, to suit different experiments |
| Display | 10.1 inch touch screen, graphical menu navigation |
| Operating system | Android |
| Programming | Built-in standard programme templates for quick file editing; on-instrument file library |
| Incubation | One-click quick incubation function |
| Power failure recovery | Automatic restart; an unfinished programme continues when power is restored |
| Connectivity | Wi-Fi |
| Data port | USB, for storing and copying PCR data and for a USB mouse |
| Screen lock | Available |
| Siting | Air channel design allows units to stand side by side |
| Supplied with | Main instrument, touch pen, power cord, 8 A fuse, instruction manual, test certificate |
Support available
- Installation & commissioning
- Calibration
- Validation & qualification (IQ/OQ/PQ)
- Operator training
- Annual maintenance contract
- Spares & consumables
Calibration is arranged through NABL-accredited laboratories under the relevant scope. We state the scope and the certificate number on the quotation — see what accreditation actually covers.
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