UV Crosslinker, 254 nm
Enclosed 254 nm ultraviolet chamber that fixes nucleic acid to hybridisation membranes under measured energy or timed control, and decontaminates PCR consumables.
Typical applications
- Cross-linking and fixing nucleic acids on hybridisation membranes
- UV sterilisation to eliminate PCR contamination
- DNA cutting in agarose gels
- RecA mutation screening
- Partial restriction endonuclease digestion of pyrimidine dimers
- UV treatment of polymers
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Add as many products as you need and send one enquiry covering all of them, or send this item on its own. Bulk and drum quantities are quoted on request.
Worth knowing before you order
Ultraviolet is the working principle of this instrument, not a hazard to be designed around: 254 nm is what forms the covalent bond between the nucleic acid and the nylon or nitrocellulose membrane, and no visible-light source will do that job. The machine is built to contain it. The sample sits in a closed stainless steel drawer, the viewing window blocks UV, and the door lock cuts the lamps the moment the drawer is opened. Keep that interlock working and never run the chamber with it defeated — unshielded 254 nm burns the cornea and the skin in seconds and the injury is not felt while it happens. Use energy mode rather than time mode for anything that has to be reproducible: it doses to a measured J/cm² instead of to a clock, which matters because lamp output falls as the tubes age. The 312 nm and 365 nm wavelengths are options and mean a different lamp set, so state the wavelength you need when the order is placed.Specification
| UV wavelength | 254 nm |
|---|---|
| Wavelength, optional | 365 nm, 312 nm |
| Energy measurement range | 0 to 99.99 J/cm² |
| Maximum UV radiation energy | 5 mW/cm² |
| Maximum irradiation area | 880 cm² |
| Lamps | 6 pcs x 8 W light tubes |
| Operating modes | UV energy mode, time mode |
| Stored settings | 50 groups of energies and times can be set and saved |
| Control | Microprocessor with a time integrator that monitors irradiation continuously and stops the lamps at the set energy |
| Display | 7 inch touch screen |
| Safety interlock | UV safety window and safety door lock; the instrument stops working automatically when the door is opened |
| Chamber | Stainless steel |
| Chamber dimensions | 350 x 290 x 140 mm |
| Loading | Drawer design, with hand grips on both sides |
| Membrane fixing time | 30 to 60 seconds |
| Packing dimensions (L x W x H) | 610 x 440 x 430 mm |
| Gross weight | 21 kg |
| Supplied with | Main instrument, power cord, instruction manual, test certificate |
Support available
- Installation & commissioning
- Calibration
- Operator training
- Annual maintenance contract
- Spares & consumables
Calibration is arranged through NABL-accredited laboratories under the relevant scope. We state the scope and the certificate number on the quotation — see what accreditation actually covers.
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Specification for Blue Light Transilluminator, 470 nm
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Full record, hazard data and enquiry for Blue Light Transilluminator, 470 nm →
Nucleic Acid Extractor, 32 Samples (DNA and RNA)
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IndentSamples per run: 1 to 32
Specification for Nucleic Acid Extractor, 32 Samples (DNA and RNA)
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Full record, hazard data and enquiry for Nucleic Acid Extractor, 32 Samples (DNA and RNA) →
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Specification for Hybridisation Oven with Tube Rotation and Shaking Base
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Full record, hazard data and enquiry for Hybridisation Oven with Tube Rotation and Shaking Base →
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Specification for ELISA Microplate Reader, 96-Well
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Full record, hazard data and enquiry for ELISA Microplate Reader, 96-Well →
