Microvolume Spectrophotometer (Biophotometer)
Cuvette-free spectrophotometer that reads nucleic acid, protein, colorimetric and OD600 absorbance from a 0.5 to 2 µL drop placed directly on the sample pedestal.
Typical applications
- DNA and RNA quantification
- Protein quantification
- OD600 cell density of bacterial cultures
- Colorimetric assays
- Fluorescence quantitation of dsDNA and RNA
- Enzyme kinetics
Add to enquiry
Add as many products as you need and send one enquiry covering all of them, or send this item on its own. Bulk and drum quantities are quoted on request.
Worth knowing before you order
The point of this instrument is that it measures a 0.5 to 2 µL drop on an open pedestal with no cuvette and no dilution, so a precious extract is wiped off and recovered rather than consumed; the short 0.02 and 0.05 mm paths let concentrated DNA be read without dilution, and the 1.0 mm path covers dilute samples. The catalogue prints no spectral bandwidth for this model anywhere, and no beam configuration either — bandwidth is the figure a pharmacopoeial method specifies, so ask for it in writing before this instrument is committed to a compendial assay. The absorbance accuracy row also carries a second clause, '±1% (7.332 Abs at 260 nm)', which cannot be reconciled with the stated absorbance range of 0.02 to 3.00 A, so it has been left out. Fluorescence measurement needs dyes and a quantitation kit; the standard accessories list carries none.Specification
| Wavelength range | 190–850 nm |
|---|---|
| Sample volume | 0.5–2 µL |
| Optical path lengths, high concentration | 0.02 mm, 0.05 mm |
| Optical path lengths, general concentration | 0.2 mm, 1.0 mm |
| Nucleic acid testing range | 2–27500 ng/µL (dsDNA) |
| Absorbance range | 0.02–3.00 A |
| Absorbance accuracy | 0.002 Abs at the 0.2 mm path |
| Light source | Xenon flash lamp |
| Detector | One UV-enhanced CMOS line array sensor |
| OD600 absorbance range | 0 to 6.000 Abs |
| OD600 stability and repeatability | (0.3) ≤ 0.5%, (3.4) ≤ 2% |
| Fluorescence sensitivity | dsDNA 0.5 pg/µL |
| Fluorescence linearity | R² > 0.995 |
| Measurement modes | Nucleic acid, protein, colorimetry, UV-Vis, OD600, fluorescence, kinetics |
| Cuvette mode | Quartz cuvettes supplied for culture-fluid work such as OD600 |
| Display and operating system | 7 in touch screen, built-in Android OS |
| Printing and data output | Built-in thermal printer; USB interface |
| Power supply | Adaptor, AC 220 V / 50 Hz |
| Packing dimensions (L x W x H) | 555 x 410 x 330 mm |
| Gross weight | 10 kg |
Support available
- Installation & commissioning
- Calibration
- Operator training
- Annual maintenance contract
- Spares & consumables
Calibration is arranged through NABL-accredited laboratories under the relevant scope. We state the scope and the certificate number on the quotation — see what accreditation actually covers.
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